We found that RUNX3 staining was mainly situated in the cytoplasm and nucleus of cancer cells in matched non-tumor tissues, while RUNX3 staining level was decreased in cancer tissues compared with matched non-tumor tissues (P <0.001, Figure 1A-1B), indicating that RUNX3 was involved in the development of HCC. Here, RUNX3 is linked to hepatocellular carcinoma.