To characterize the regulatory cascade involving WWOX, bcl-2, OPN, RUNX2, and VEGF in osteosarcoma cells, we transfected MG-63 cells overexpressing WWOX with bcl-2 or RUNX2 expression plasmids and measured WWOX, bcl-2, OPN, RUNX2, and VEGF expression using Western blotting. This evidence concerns the gene SPP1 and osteosarcoma.