To determine whether WWOX regulates bcl-2, RUNX2, VEGF, and OPN expression in osteosarcoma cells, we used Western blotting and qRT-PCR to examine protein and mRNA levels of these factors in U2OS, SAOS2, and MG-63 cells with WWOX overexpression or knockdown. This evidence concerns the gene SPP1 and osteosarcoma.