Finally, two cases of lymph node metastasis are presented in Fig. 2J–O, where HUGL-1 is partially retained at the membrane (Fig. 2J, arrow indicates an example region) or completely cytoplasmic (Fig. 2M); YAP (Fig. 2K,N) and c-MYC (Fig. 2L,O) are highly expressed in the cancer cell nuclei, and Cas3 stains dispersed stromal cells (arrowheads i.s. in Fig. L,O). Here, EFS is linked to metastatic malignant neoplasm in the lymph nodes.