Finally, two cases of lymph node metastasis are presented in Fig. 2J–O, where HUGL-1 is partially retained at the membrane (Fig. 2J, arrow indicates an example region) or completely cytoplasmic (Fig. 2M); YAP (Fig. 2K,N) and c-MYC (Fig. 2L,O) are highly expressed in the cancer cell nuclei, and Cas3 stains dispersed stromal cells (arrowheads i.s. in Fig. L,O). The gene discussed is YAP1; the disease is cancer.