To gain a better understanding of SALL4 requirement in normal hematopoiesis, we then injected 8-week-old Sall4f/f/CreERT2, Sall4f/f/Cre-, and RosaCreERT2 control mice with tamoxifen and verified Sall4 excision as we did with the MLL-AF9 AML model. This evidence concerns the gene MLLT3 and acute myeloid leukemia.