To clarify the role of CLCA1 action in CRC tumorigenesis and metastasis, we constructed stable CLCA1-upregulated (CLCA1-ACT) and CLCA1-knockout cells (CLCA1-KO) in the SW620 cell line, as well as the respective negative control cells (CLCA1-ACT-NC and CLCA1-KO-NC), using CRISPR/Cas9 plasmids (Fig. 3a, b and c). Here, CLCA1 is linked to colorectal carcinoma.