In the first month after diabetes induction, Slit2 was expressed in the ganglion cell layer, nerve fiber layer, the inner nuclear layer and the outer nuclear layer (Fig 3B), while Robo1 positive staining was observed in the ganglion cell layer, the nerve fiber layer, the inner nuclear layer, the inner plexiform layer and the inner segment of the photoreceptor layer (Fig 4B). Here, ROBO1 is linked to diabetes mellitus.