The hotspot of BRAF mutations in CRC is substitution from valine to glutamic acid at codon 600 (V600E), located in exon 15, leading to 130- to 700-fold increased BRAF kinase activity compared with that of wild-type BRAF; these mutations are reported in ∼5–12% of cases (Davies et al, 2002; Andreadi et al, 2012). The gene discussed is BRAF; the disease is colorectal carcinoma.