Toll-like receptor 4 is the main receptor that detects gut-derived endotoxins and regulates hepatic inflammation in NASH and probiotics were shown to decelerate the progress of NAFLD by inhibiting the LPS-TLR4-signaling pathway, improving intestinal flora dysbiosis, restoring normal gut homeostasis, and upregulating expression of tight junction proteins strengthening the gut barrier (44, 48). The gene discussed is TLR4; the disease is metabolic dysfunction-associated steatotic liver disease.