ESR1 and breast cancer: The extract inhibited growth of breast cancer cell lines MCF-7 (ER+, HER2/neu−), MDA-MB-231 (ER−, HER2/neu−), and MDA-MB-453 (ER−, HER2/neu+) and induces apoptosis in MDA-MB-453 cells by depolarizing mitochondrial membrane potential and releasing cytochrome C into the cytosol, and thus triggered programmed cell death through apoptosis [77].