No significant differences were detected in p21, p16, Lamin B1 and GLB1 mRNA (Additional file 3: Figure S1A) or protein levels (Additional file 3: Figure S1B) between glial cells unexposed or exposed to GBM CM, suggesting that glial cells are not undergoing senescence in response to GBM CM. This evidence concerns the gene LMNB1 and glioblastoma.