FOXO3 and neoplasm: To determine whether the anti-tumor effects of aplysin depended on FOXO3a, cells were transfected with four LV3 lentiviral constructs expressing a short hairpin targeting FOXO3a (shFOXO3a-1, shFOXO3a-2, shFOXO3a-3 and shFOXO3a-4), LV3 was used as empty vector control.