To better understand the T4-induced autophagy in A549 and A549/DDP lung cancer cells through the inhibition of the PI3K/AKT/mTOR signaling pathway, the effects of three inhibitors (wortmannin, perifosine and rapamycin for PI3K, AKT and mTOR, respectively) on the PI3K/AKT/mTOR signaling pathway were evaluated. This evidence concerns the gene AKT1 and lung cancer.