Master regulators of reduced gene expression were highly conserved, with 8 TFs (EP300, HNF4G, HDAC2, NR2F2, FOXA1, HNF4A, CEBPB, and FOXA2) identified in the Oncopig and all human HCC cell lines (Table 1). This evidence concerns the gene HNF4A and hepatocellular carcinoma.