The results showed that CK19/GPC3 expression pattern [hazard ratio (HR)=1.782, 95% confidence interval (CI)=1.137–2.793, P=0.012], histological grading (HR=1.969, 95%CI=1.292–3.001, P=0.002) and tumor number (HR=2.173, 95%CI=1.328–3.557, P=0.002) were the independent predictors of RFS. This evidence concerns the gene GPC3 and neoplasm.