Using the Kaplan–Meier method, univariate analysis showed that microvascular invasion (P<0.01), macroscopic tumor thrombi (P<0.05), moderate vs poor histological grading (P<0.01), CK19/GPC3 expression pattern (overall: P<0.01; within groups: CK19−/GPC3− vs CK19+/GPC3+: P<0.01, CK19−/GPC3+vsCK19+/GPC3+:P<0.01) and tumor number (overall: P<0.01; within groups both single vs two and single vs three showed P<0.01) were significantly associated with the RFS of patients (Table 1). The gene discussed is GPC3; the disease is neoplasm.