ANXA5 and Familial prostate cancer: In attempt to evaluate whether the exposure of b-AP15 (0–2 μM) to human prostate cancer cells induces cell death, Annexin-V FITC and propidium iodide (PI) double staining were applied on LNCaP and PC3 living cells and detected with fluorescence microscopy (Figure 3A) or quantified by flow cytometry (PC-3 cells; Figure 3B and 3C).