In the present study, we depleted Foxp3+ Tregs in infected Irf8-/- mice using repeated treatments with an anti-CD25 mAb prior to infection, on the day of infection and day 7 p.i. Consistent with previous studies, there were significant increases in Th2 cytokines as well as IL-6 compared to isotype control, infected Irf8-/- mice, but there were no effects on the adult worm burden or the numbers of CD4+GATA3+ T cells or AAMØ [35, 38, 39]. Here, IRF8 is linked to infection.