In contrast, the unmanipulated HBV transgenic controls showed no serum ALT elevation, indicating no liver disease for the entire experimental period, as previously observed.[10] In addition, we have previously observed that sham-treated HBV transgenic mice which had been thymectomized, irradiated, and reconstituted with immunologically tolerant transgenic donor bone marrow cells and splenocytes developed no liver disease or no liver tumors.[10, 11]. Here, GPT is linked to liver disorder.