IL-8 is a proinflammatory cytokine that functions as a chemoattractant and is also a potent pro-angiogenic factor.29 It has been reported that the IL-8 level in the serum of cancer patients was highly elevated during tumor progression and contributed to the development of distant metastasis.30, 31 Given the above data that HT-29 CM promoted the transition of NECs toward TECs, we hypothesized that IL-8 in the tumor microenvironment might have a prominent role in this process. This evidence concerns the gene CXCL8 and neoplasm.