GCLC and neoplasm: We next assayed the expression of three canonical coding components of the antioxidant programme orchestrated by NFE2L2 to suppress reactive oxygen species (ROS) accumulation in cells, all of which were detected as NFE2L2 activated in the tumour data (Supplementary Data 2): GCLC, crucial for synthesis of the antioxidant glutathione; NQO1, which prevents production of ROS from quinones and TXNRD1, which catalyses reduction of the antioxidant thioredoxin from the oxidized to the reduced form67.