Hence, RT-PCR analysis was‏ performed and the mRNA profile of sall4 has demonstrated‏ that chrysin exerts cytotoxicity on CT26 colon‏ tumor cells via down regulation of the sall4 expression.‏ ‏ However, sall4 down regulation could be considered‏ as a palliative marker for treatment of colon cancer. This evidence concerns the gene SALL4 and malignant colon neoplasm.