Using two human ICC cell lines, KKU-M213 and HuCCA-1, we demonstrate that h-TGF-β1 promotes cell invasion and that inhibition of ERK1/2 pathway suppresses this effect in both ICC cell lines with KKU-M213 being more sensitive to ERK1/2 inhibition. Here, TGFB1 is linked to intrahepatic cholangiocarcinoma.