To assess whether the top KD subnetworks were enriched for previously known disease genes that mostly represent the strong and replicated genes as a means of cross-validation, we manually curated previously reported genes associated with CVD, T2D, and intermediate metabolic traits related to CVD, T2D (glucose, insulin, lipids, obesity) from DisGeNET [32] and the NHGRI GWAS Catalog [6] (Fig 1C, genes listed in S3 Table). The gene discussed is INS; the disease is obesity due to melanocortin 4 receptor deficiency.