These results were then validated in a second study-set of 160 tumors confirming the presence of subtype-specific mutations in several genes such as PIK3CA in myxoid/round cell liposarcoma, TP53 in pleomorphic liposarcoma, and NF1 in myxofibrosarcoma and pleomorphic liposarcoma. This evidence concerns the gene PIK3CA and pleomorphic liposarcoma.