Furthermore, testosterone was shown to stimulate cardiac myocyte differentiation from mouse embryonic stem cells and P19 embryonic carcinoma cells through AR-regulated transcription involving ARE regions of MEF2C-regulated genes, which led to an increase in histone acetylation in the DNA (Di-Luoffo et al., 2015), and the MADS domains of MEF2 were reported to potentially generate regulatory protein–protein interactions for MEF2C and AR (Black and Olson, 1998). This evidence concerns the gene AR and embryonal carcinoma.