To investigate the effects of two different FAK inhibitors (PF-562271 and PF-573228) on the growth rate inhibition and the levels of apoptosis on colorectal cancer cells, HCT116 and SW480 were treated with different concentrations of FAK-I (0.1, 1, 10, 20 and 50 μM) for 12 h and a nuclear staining assay with DAPI (4, 6-Diamidino-2-phenylindole, Sigma) was performed. This evidence concerns the gene PTK2 and colorectal cancer.