Next, we tested the cytotoxic activity of ENDOS/ADC in four human tumor cell lines expressing different levels of endosialin: high (osteosarcoma SJSA-1), intermediate (neuroblastoma SKNAS and Ewing's sarcoma A-673), or not expressing endosialin (metastatic A375 melanoma cells) (Figure 2D upper panel). This evidence concerns the gene CD248 and osteosarcoma.