Next, in order to test if IL-8 is a key factor in the crosstalk between tumor cells and fibroblasts or macrophages in the regulation of TNBC cell proliferation and migration, we performed MDA-MB-231 cell proliferation and migration assays with conditioned media of fibroblasts or macrophage induced by TCM of TNBC cells in which the IL-8 is neutralized with an anti-IL-8 specific antibody. The gene discussed is CXCL8; the disease is neoplasm.