To characterize the seven NSCLC-derived BRAF mutations identified in our clinical samples and to test the sensitivity of a broader subset of non-V600 BRAF mutations to ERK pathway inhibitors, we generated 13 BRAF-expressing plasmids (in our cohort, supplemented by NSCLC mutations reported by others, recombinant BRAF proteins are 3X flag-tagged and are referred to as flag-BRAF for simplicity). The gene discussed is BRAF; the disease is non-small cell lung carcinoma.