ZNF516 and neoplasm: However, the growth of the primary tumor and metastasis were significantly enhanced in mice implanted with MDA-MB-231-Luc-D3H2LN tumors overexpressing ZNF516 + EGFR compared with tumors overexpressing ZNF516 and were significantly inhibited in mice implanted with MDA-MB-231-Luc-D3H2LN tumors carrying shZNF516 + shEGFR compared with tumors carrying shZNF516 (Fig. 8a, b).