The results showed that overexpression of ZNF516 suppressed the growth of the primary tumor and the metastasis of the MDA-MB-231-Luc-D3H2LN tumors to lung or liver, whereas ZNF516 depletion promoted the growth of the primary tumor and the metastasis of the MDA-MB-231-Luc-D3H2LN tumors to lung, liver, and spleen (Fig. 8a, b). The gene discussed is ZNF516; the disease is neoplasm.