To investigate the role of ZNF516 in the growth and metastasis of breast cancer in vivo, MDA-MB-231 cells engineered to stably express firefly luciferase (MDA-MB-231-Luc-D3H2LN) were infected with lentiviruses carrying ZNF516 or/and EGFR, or infected with lentiviruses carrying shZNF516 or/and shEGFR. This evidence concerns the gene EGFR and breast carcinoma.