In our data, in MS monocytes, the frequency of the TNFα+ classical monocyte subset was similar to that of the same subset in HC monocytes, but the TNFα+ intermediate monocyte subset was upregulated by CD40L stimulation, being significantly different from the HC intermediate monocyte subset (p < 0.01) (Fig. 5b). This evidence concerns the gene CD40LG and myeloid sarcoma.