Thus, to investigate the frequency of the three different monocyte subsets, peripheral blood mononuclear cells (PBMCs) from HC, MS, and NMOSD patients (n = 20 each) were stained for CD3, CD14, CD16, CD19, CD56, and CD66b to gate the monocyte subset (Additional file 1: Figure S2). This evidence concerns the gene NCAM1 and myeloid sarcoma.