Similarly, colony formation assay results revealed that clonogenic survival was increased following transfection with upregulated lnc-IGFBP4–1 in PC9 cells (Fig. 3b), while declined in sh-lnc-IGFBP4–1 transfected GLC-82 cells (Fig. 4b).We next examined whether the tumor cell cycle was affected after lnc-IGFBP4–1 upregulatiom or lnc-IGFBP4–1 knock-down by Flow cytometry assay. This evidence concerns the gene IGFBP4 and neoplasm.