Moreover, we analyzed mRNA expression levels in patients with colon cancer, and results showed that expression of PODXL was positively correlated with mesenchymal markers such as vimentin (VIM), N-cadherin (CDH2), Twist2, Slug (SNAI2), and Zeb1, whereas it was negatively correlated with epithelial marker E-cadherin (CDH1) expressions (Figure 4D). Here, TWIST2 is linked to malignant colon neoplasm.