To confirm the apoptotic effect of NCKU-21 in the NSCLC cell lines, we evaluated the membrane translocation of PS, an early marker of cell apoptosis, and activity change of caspase-3, a critical executive caspase required for DNA fragmentation, in A549 and CL1-5 cells incubated with 2 μM of NCKU-21. Here, CASP3 is linked to non-small cell lung carcinoma.