2011), its underlying mechanism remains unclear. To address this, we next investigated the phenotype provoked by RNAi‐mediated depletion of CHAMP1 in human cancer cell line. Using siRNAs targeting different sequences of CHAMP1 ORF (open reading frame), we confirmed that the depletion of CHAMP1 induced chromosome misalignment in metaphase, which was further confirmed in different human cell lines, HeLa and U2OS cells (Fig. 2A–C). These results are consistent with the previous observation that CHAMP1 is important for proper chromosome alignment during mitosis (Itoh et al. 2011). This evidence concerns the gene CHAMP1 and cancer.