Cytokine and apoptotic marker detections in response to AML blasts revealed that pre-incubation of NK cells from expansion period 10–14 days with 1 μg/ml TBs resulted in elevated levels of perforin and GraA and GraB without changes in the amount of TNFα, IFNγ, and granulysin (Figure 2D). Here, PRF1 is linked to acute myeloid leukemia.