In Western blot analysis, the expression of cell cycle-related proteins (cyclin D1, cyclin D3 and CDK4) decreased and was completely suppressed after 48 h of MKH-DMG treatment in both DCP-positive (PLC/PRF/5 and Hep3B) and DCP-negative (SK-Hep-1) HCC cell lines. The gene discussed is CCND1; the disease is hepatocellular carcinoma.