To investigate the impact of HD‐RNAi on suppression of Pt infection in advanced generations, three transgenic wheat lines for each of the hp‐PtMAPK1RNAi (MAPK1‐2163, MAPK1‐2169 and MAPK1‐2166) and hp‐PtCYC1RNAi (CYC1‐2236, CYC1‐2248 and CYC1‐2224) construct that showed enhanced disease protection were selected and selfed to obtain T2 progeny. This evidence concerns the gene HP and Huntington disease.