As shown in Figure 3A and B, miR‐206 dramatically repressed the expression of CDK9 at mRNA (Bell7402, P = 0.0014; HepG2 P = 0.0222) and protein (Bell7402, P = 0.011; HepG2, P = 0.0156) levels in HCC cells, which were markedly attenuated by cotransfection of miR‐206 inhibitor (Bell7402, P = 0.0393; HepG2, P = 0.0026) (Fig. 3A and B) or GV230‐CDK9 without 3′ UTR of CDK9 (Bell7402, P = 0.0415; HepG2, P = 0.0072) (Fig. 3C and D). This evidence concerns the gene CDK9 and hepatocellular carcinoma.