PPP1R12C and cervical carcinoma: To augment the stringency of the genotyping assay, we next carried out dose–response experiments in human cervix carcinoma HeLa cells using increasing amounts of adenoviral vectors encoding either Cas9 or Cas9D10A, each mixed with a fixed amount of an adenoviral vector expressing a gRNA addressing each Cas9 protein to AAVS1. A direct relationship between the detection of small insertions and deletions (indels) and nuclease concentrations could be readily established after Cas9:gRNAS1 delivery, whereas this was much less so upon Cas9D10A:gRNAS1 transfer (Supplementary Fig. 1b).