Lastly, the incorporation assays as well as CCK-8 assays and transwell assays were re-executed to finally determine whether MALAT1 could regulate ROCK1/ROCK2 and their mediated proliferation/metastasis via a ceRNA of miR-144-3p in osteosarcoma cells MNNG/HOS. The gene discussed is MALAT1; the disease is osteosarcoma.