In order to investigate a possible role of PATZ1 in downregulating CXCR4 gene expression in GBM cells, we transiently transfected the VS-GB cells, a GBM-derived primary cell line that does not express PATZ1, with a plasmid expressing PATZ1, and measured CXCR4 mRNA and protein levels by qRT-PCR and western blot, respectively (Figure 7D). Here, CXCR4 is linked to glioblastoma.