At last, we verified five methylated sites (cg06363129, cg07220448, cg11417025 in SOSTDC1; cg08843517 in CYBA; and cg05385513 in EFEMP1) in PCa and normal tissues, including adjacent tissues and benign prostatic hyperplasia (BPH) through pyrosequencing (Figure 5). This evidence concerns the gene SOSTDC1 and benign prostatic hyperplasia.