At last, we verified five methylated sites (cg06363129, cg07220448, cg11417025 in SOSTDC1; cg08843517 in CYBA; and cg05385513 in EFEMP1) in PCa and normal tissues, including adjacent tissues and benign prostatic hyperplasia (BPH) through pyrosequencing (Figure 5). The gene discussed is EFEMP1; the disease is benign prostatic hyperplasia.