To ascertain that the CD3xPDL1 BiTE binds to PDL1 on cells, C8161 human melanoma cells that constitutively express PDL1 and PDL1- MEL1011 cells were incubated with BiTE or an irrelevant his-tagged recombinant protein (TROY-Fc) followed by fluorescently tagged anti-his antibodies (Figure 2A). The gene discussed is CD274; the disease is melanoma.