LDHA and breast cancer: In order to assess the metastatic ability of ALDH-deficient cell populations in vivo, GFP-labeled MDA-MB-468 cell populations (468CON, 468ALDH1A1low, 468ALDH1A3low cells) or CMFDA-labeled SUM159 cell populations (159CON, 159ALDH1A1low, 159ALDH1A3low cells) were inoculated on the CAM of 9- or 12-day-old chicken embryos, and the percentage of breast cancer cell extravasation into the CAM and formation of micrometastases in the chicken embryo were analyzed (Figure 4).