We used primers for the gene sequences of UBB (references), ESR1 (for luminal or ER/PR+ cancer cells), HER2 and GRB7 (for HER2+ cancer cells), EPCAM, KRT7, KRT8, KRT18, and KRT19, all of which have been commonly discussed for the purpose of breast cancer diagnosis [7, 25], [26]. The gene discussed is GRB7; the disease is breast cancer.