We also examined the ratio levels after the diagnosis of metastatic disease prior to the start of palliative chemotherapy both in the available samples from the study population (nine tissue HER-2-positive and eight tissue HER-2-negative patients) and in the extra samples (14 tissue HER-2-positive and eight tissue HER-2-negative patients), in total 39 patients: 23 tissue HER-2-positive and 16 tissue HER-2-negative. Here, ERBB2 is linked to metastatic neoplasm.