With higher oleiferasaponin A1 concentration, the insulin levels of RIN-m5f (islet-β cells) was enhanced, which indicates that oleiferasaponin A1 has potential hypoglycemic activity against the damage induced by high glucose, and oleiferasaponin A1 may be a therapeutic agent for hyperglycemia treatment. The gene discussed is INS; the disease is Hyperglycemia.