Interferon-γ (IFN-γ, a Th1 cytokine), interleukin-4 (IL-4, a Th2 cytokine), interleukin-5 (IL-5, a Th2 cytokine), interleukin-10 (IL-10, a Treg cytokine), interleukin-17 (IL-17, a Th17 cytokine) and tumor necrosis factor-α (TNF-α, a proinflammatory cytokine) were measured to confirm the successful establishment of mice asthma model. Here, TNF is linked to asthma.