TFF3 and pachyonychia congenita: Furthermore, matched RNAseq and 450K data were available for 494 PC and 35 AN tissue samples [38] and demonstrated a significant inverse correlation between RNA expression and TFF3 promoter methylation at two out of three CpG sites (cg21970261: Spearman’s ρ = −0.406, p < 0.001, Figure 2C; cg14283447: Spearman’s ρ = −0.295, p < 0.001, Figure S3B; cg04806409, p > 0.05, Figure S2A), thereby corroborating and expanding our previous findings in a small set of prostatic cell lines [36], and together suggesting that epigenetic changes are associated with TFF3 upregulation in PC.