Logistic regression analysis indicated that age (older than 65 years old), male sex, albumin ≤ 4 g/dL, prothrombin time ≤70%, AFP concentration ≥ 20 ng/mL, PIVKA-II concentration ≥ 40 mAU/mL, and minor genotype of the MICA SNP were independent factors that were significantly associated with HCC development (Table 2). This evidence concerns the gene AFP and hepatocellular carcinoma.